3. Building biological profiles

This chapter moves from theory to practice by predicting the “anatomy” of the protein at the level of its biophysical properties. Using the B2BTools suite, we will explore how different regions of the protein behave in terms of dynamics, structure, and flexibility.

Chapter questions

How to use the B2BTools online platform to predict biophysical profiles for protein sequences ?
How to find biophysical patterns using the platform ?

3.1 Biophysical profiles

This package ¹ offers biophysical feature predictors for protein sequences as well as different file parses and other utilities to help you with your protein data analysis. If your input data consists on one or more sequences not aligned, we provide you with the Single Sequence mode. On the other hand, if your input is a Multiple Sequence Alignment (MSA), we provide the MSA mode. For NMR data, we have the predictor ShiftCrypt (out of the scope of this course).

Predictor	Description	Publication
`DynaMine` ²	Fast predictor of protein backbone dynamics using only sequence information as input. The version here also predicts side-chain dynamics and secondary structure predictors using the same principle.	doi: 10.1038/ncomms3741
`DisoMine` ³	Predicts protein disorder with recurrent neural networks not directly from the amino acid sequence, but instead from more generic predictions of key biophysical properties, here protein dynamics, secondary structure and early folding.	doi: 10.1016/j.jmb.2022.167579
`EFoldMine` ⁴	Predicts from the primary amino acid sequence of a protein, which amino acids are likely involved in early folding events.	doi: 10.1038/s41598-017-08366-3
`AgMata` ⁵	Single-sequence based predictor of protein regions that are likely to cause beta-aggregation.	doi: 10.1093/bioinformatics/btz912
`PSPer` ⁶	PSP (Phase Separating Protein) predicts whether a protein is likely to phase-separate with a particular mechanism involving RNA interacts (FUS-like proteins). It will highlight the regions in your protein that are involved mechanistically, and provide an overall score.	doi: 10.1093/bioinformatics/btz274
`Shift Crypt` ⁷	Auto-encoding NMR chemical shifts from their native vector space to a residue-level biophysical index	doi: 10.1093/nar/gkaa391

3.1.1 DynaMine

DynaMine predicts local protein backbone dynamics directly from the amino acid sequence. More specifically, it estimates how rigid or flexible each residue is expected to be in solution, based on fast (nanosecond-scale) backbone motions.

The predictor was trained on backbone order parameters derived from NMR chemical shift data, which reflect protein behaviour in solution rather than a single static structure. Because of this, DynaMine captures intrinsic conformational tendencies that are independent of a specific folded state.

The output is a per-residue numerical score:

Higher values indicate rigid regions with a strong tendency to adopt stable conformations
Lower values indicate flexible or dynamic regions

Backbone dynamics are not equivalent to intrinsic disorder. A residue may be flexible while still being part of a folded structure, and rigid residues are not guaranteed to be structured under all conditions. DynaMine scores should therefore be interpreted as biophysical propensities, not as absolute structural assignments.

Specifically, for the backbone propensity prediction, the range values are:

Values > 1.0: Membrane spanning regions
Values > 0.80 and < 1.0: Rigid conformations
Values > 0.69 and < 0.80: Context dependent
Values < 0.69: Flexible regions

Backbone plot example

P07949 on Scop3P — DynaMine backbone dynamics for P08100

3.1.2 DisoMine

DisoMine predicts intrinsic disorder, defined as regions of a protein that do not adopt a stable three-dimensional structure under physiological conditions.

The model was trained on experimentally annotated disordered regions from curated datasets and predicts disorder at the residue level. Unlike many disorder predictors, DisoMine does not rely directly on amino acid composition or evolutionary profiles. Instead, it uses predicted biophysical properties—such as backbone dynamics, secondary structure propensity, and early folding behaviour—as input.

The output is a probability score between 0 and 1:

Higher values indicate a higher likelihood that the residue is intrinsically disordered
Lower values indicate a tendency toward structured behaviour

DisoMine is particularly effective at identifying long disordered regions. The predictions are probabilistic and context-dependent: a high disorder score does not imply permanent disorder in all conditions, but rather a strong tendency to remain flexible or unstructured.

The threshold for this prediction is:

Above 0.5: likely a disordered position
Below: unlikely a disordered position

Disorder plot example

DisoMine disorder propensities for P08100

3.1.3 EFoldMine

EFoldMine predicts early folding residues, defined as amino acids that are likely to form stable local structure at the very beginning of the protein folding process.

The predictor was trained using experimental data derived from hydrogen–deuterium exchange experiments, which identify residues that gain protection early during folding. EFoldMine therefore focuses on folding kinetics, rather than final structure.

The output highlights residues with high early folding propensity:

These residues are likely to initiate local structure formation
They may play a key role in guiding the folding pathway

Early folding residues are not necessarily rigid, conserved, or part of the final folded core. EFoldMine does not predict folding rates or final stability, but rather identifies sequence-encoded folding initiation signals that act early in the folding process.

The threshold for this prediction is:

Values > 0.169: indicate residues that are likely to start the protein folding process
Below: indicate residues that are unlikely to start the protein folding process

EFoldMine plot example

3.1.4 AgMata

AgMata predicts beta-sheet aggregation propensity from single protein sequences. It identifies regions that are intrinsically prone to form intermolecular beta-sheet interactions, a key feature of amyloid-like aggregation.

Unlike many aggregation predictors, AgMata is not directly trained on known aggregating sequences. Instead, it uses a generalized statistical potential that integrates predicted biophysical properties, including backbone dynamics and secondary structure tendencies.

The output highlights regions with increased aggregation risk:

Higher values indicate a stronger intrinsic tendency toward beta-aggregation
Lower values indicate reduced aggregation propensity
Peaks indicate residues likely to be involved in beta-sheet aggregation.

AgMata predictions reflect intrinsic sequence-level risk, not actual aggregation outcomes. Cellular context, protein concentration, interaction partners, and chaperones are not considered. The predictor is most informative when interpreted together with disorder and dynamics predictions.

AgMata plot example

AgMata takes longer to predict the peaks

Some of the activities will skip this predictor due to long waiting times.

3.2 Extracting the protein sequence

The first step is to download the protein sequence in FASTA format.

Hands-on: P07949 canonical sequence

Download the canonical protein sequence from UniProtKB.
Visit the official UniProtKB website, locate the protein entry.
Download the FASTA sequence from the contextual menu.

Solution

The following FASTA sequence corresponds to the protein of interest and was retrieved from https://rest.uniprot.org/uniprotkb/P07949.fasta:

>sp|P07949|RET_HUMAN Proto-oncogene tyrosine-protein kinase receptor Ret OS=Homo sapiens OX=9606 GN=RET PE=1 SV=3
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAP
EEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRSLDHSSWEKLSVRNRGFPLLT
...
DGTNTGFPRYPNDSVYANWMLSPSAAKLMDTFDS

You should now have the file P07949.fasta available in your working environment.

./training-data-directory
└─── P07949.fasta

3.3 Analysing single sequences (standalone mode)

As mentioned earlier, the B2BTools suite provides several predictors to analyse protein biophysical properties:

Backbone and side-chain dynamics (via DynaMine)
Intrinsically disordered residues (via DisoMine)
Early folding regions (via EFoldMine)
Beta-aggregation-prone regions (via AgMata)

The Bio2Byte lab provides an online platform ⁸ to run the biophysical predictions described above for proteins of interest. The platform is available at: https://bio2byte.be/online_predictors/ (this resource is still under active development, and user feedback is highly appreciated. A previous version of the platform is available at: https://bio2byte.be/b2btools).

Attention: Required token

First of all, write down in your notes a personal alphanumeric TOKEN to enable job submission and later retrieval of results on the B2BTools platform.

Access the B2BTools platform and set your token.
The token will be saved in your browser internal storage and remembered every time you access again the website.

Where is the TOKEN textbox?

Token input example — Notice the input textbox for your TOKEN

Hands-on: Predicting the biophysical features

Select the Single Sequence analysis type and upload your FASTA file.
Submitted jobs can be accessed from the Token results page: https://bio2byte.be/online_predictors/past-results/.
Locate your job and open the corresponding results page.

Solution

You will be able to find the results inside the “Single Sequence Predictions” table.
Your job will have an action button “View Results” that opens a link like https://bio2byte.be/online_predictors/results/UUID/.

3.3.1 Understanding the results as biological profiles

3.3.1.1 Visualization of biophysical profiles

Explore the overview predictions

3.3.1.2 Exploring intrinsic flexibility and folding propensity

Explore dynamics

Explore Early folding events

Explore Disorder

Explore Beta-Aggregation propensities

Interactive results

For further details: Open the results on the B2BTools platform.

☕️ COFFEE BREAK

Time to grab a coffee or tea. We’ll be back in ten minutes…

3.4 Multiple sequence alignments (MSA)

To fully exploit the predictive power of biophysical tools, it is useful to study the target protein in the context of related proteins. Comparing similar sequences allows the identification of conserved biophysical profiles as well as positions that show divergence across homologs.

3.4.1 Working with the aligned kinase domain

In the following hands-on activities the focus will be on the homolog kinase domains of the P07949’s 90%-similarity proteins.

3.4.1.1 Fetching the similar proteins

UniProtKB provides access to similar proteins directly from the protein entry page, in the Similar proteins section.

Hands-on: Getting the 90%-identity protein sequences

Open the protein entry in UniProtKB and locate the Similar proteins table at: https://www.uniprot.org/uniprotkb/P07949/entry#similar_proteins.

Select the entries with 90% sequence identity and choose to view all results in UniProtKB.

Exclude other human proteins and short sequences to retain proteins with lengths between 1000 and 1120 amino acids.
Once the entries are selected, download the canonical sequences using the Download option in FASTA format, without compression.

Before proceeding with the alignment, add the P07949 sequence at the top of the downloaded FASTA file.

Solution

The canonical sequences can be retrieved from the following UniProtKB REST endpoint.

The FASTA file contains all the sequences:

>tr|A0A0D9REZ9|A0A0D9REZ9_CHLSB Proto-oncogene tyrosine-protein kinase receptor Ret OS=Chlorocebus sabaeus OX=60711 GN=RET PE=3 SV=1
LTSLLPTVALGLYFSRDAYWEKLYVDQPAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYR
TRLHENNWICIQEDTGLLYLNRSLDRSSWEKLSGRNRGFPLLTVYLKVFLSPTFLREGEC
...
YANWMLSPSAAKLMDTFDS

>tr|A0A2K5MKU6|A0A2K5MKU6_CERAT Proto-oncogene tyrosine-protein kinase receptor Ret OS=Cercocebus atys OX=9531 GN=RET PE=3 SV=1
MAKATSGAAGLRLLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQPAGTPLLYVHALRDS
PEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRSLDHSSWEKLSGRNRGFPLL
...
ADGTNTGFPRYANDSVYANWMLSPSAAKLMDTFDS
...

>tr|H9ZC00|H9ZC00_MACMU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Macaca mulatta OX=9544 GN=RET PE=2 SV=1
MAKATSGAAGLRLLLLLLLLPLLGKGALGLYFSRDAYWEKLYVDQPAGTPLLYVHALRDA
PEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRSLDRSSWEKLSGRNRGFPLL
...
ASTPSDSLLYDDGLSEEETPLVDCNNAPLPRALPSTWIENKLYGRISHAFTRF

You should now have the file P07949.90-similar.fasta available in your working environment.

./training-data-directory
│─── P07949.fasta
└─── P07949.90-similar.fasta

3.4.1.2 Building the MSA

To continue this activity, the multiple sequence alignment (MSA) will be generated using the online Clustal Omega server provided by EMBL-EBI.

Hands-on: Aligning the sequences

Open the Clustal Omega submission form at: https://www.ebi.ac.uk/jdispatcher/msa/clustalo.
Upload the file P07949.90-similar.fasta as input.
Request the output format as Pearson/FASTA and keep the original sequence order by setting the order option to input.
Set the job title to P07949.90-similar.msa.

Solution

After the job has completed on the EMBL-EBI servers, you will be redirected to the results page.
This page contains several tabs for exploring the alignment.

From the Tool output tab, download the MSA by clicking the blue Download button.

Example of the aligned P07949 protein sequence:

>sp|P07949|RET_HUMAN Proto-oncogene tyrosine-protein kinase receptor Ret OS=Homo sapiens OX=9606 GN=RET PE=1 SV=3
MAKATSGAAGLRLLLL--LLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRD
...
LKQVNHPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSS
...
LTRADGTNTGFPRYPNDSVYANWMLSPSAAKLMDTFDS

You should now have the file P07949.90-similar.msa.fasta available in your working environment.

./training-data-directory
│─── P07949.fasta
│─── P07949.90-similar.fasta
└─── P07949.90-similar.msa.fasta

3.4.1.3 Extracting the domain of interest

The next step is to focus the analysis on the kinase domain positions across all aligned sequences. For this purpose, the course provides a Google Colab notebook that runs a Python script to identify and extract the aligned positions corresponding to a defined amino acid range in the original reference sequence.

Attention: Google Account required

You must log in using a Google account to run the code in this notebook. By default, you can use your @gmail.com address.

Hands-on: Extracting the aligned domain from the MSA

Open the Google Colab notebook related to this activity.
The notebook is divided into two main sections.
First, run the four hidden cells in the section Dependencies and method definitions by clicking the Run icon. This step loads the required Python libraries and helper functions into memory.
Next, move to the Usage section and execute the cells in order.
The first cell will prompt you to upload the alignment file P07949.90-similar.msa.fasta.
After the file is uploaded, fill in the fields in the Extraction parameters cell and click the Run icon to start the extraction.
Once the execution is complete, download the output file from the Files panel to your local working environment.

Solution

Use the following parameters in the Extraction parameters cell, based on the kinase domain definition:
- uniprot_id: P07949
- start_pos: 724
- end_pos: 1016
- max_gap_fraction: 0.4 (40%)
- output_filename: P07949.90-similar.msa.kinase.fasta
Save the file from the Files panel on the left navigation (folder icon), locate the file, open the contextual menu, and select Download.

Output console message

After running the cell, a new file will be generated and a summary message similar to the one below will be displayed:

Parameters:
UniProt ID: P07949
Start Position: 724
End Position: 1016
Max Gap Fraction: 0.4
For sp|P07949|RET_HUMAN: found 0.006779661016949152 as gap fraction (seq len: 295)
For tr|A0A0D9REZ9|A0A0D9REZ9_CHLSB: found 0.0 as gap fraction (seq len: 295)
...
File P07949.90-similar.msa.kinase.fasta written using alignment columns: 725 and 1020 (295 residues)

Kinase sequences

The extracted alignment will start as follows:

>sp|P07949|RET_HUMAN Proto-oncogene tyrosine-protein kinase receptor Ret OS=Homo sapiens OX=9606 GN=RET PE=1 SV=3
LVLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHP
...
NLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYL

>tr|A0A0D9REZ9|A0A0D9REZ9_CHLSB Proto-oncogene tyrosine-protein kinase receptor Ret OS=Chlorocebus sabaeus OX=60711 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQDAPGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHP
...
NLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL

You should now have the file P07949.90-similar.msa.kinase.fasta available in your working environment.

./training-data-directory
│─── P07949.fasta
│─── P07949.90-similar.fasta
│─── P07949.90-similar.msa.fasta
└─── P07949.90-similar.msa.kinase.fasta

3.4.1.4 Predicting the aligned biophysical features

We are now ready to predict the aligned biophysical features using B2BTools.

Hands-on: Predicting the aligned biophysical features for the kinase domain

Open a new browser tab and go to the online platform at: https://bio2byte.be/online_predictors
This time, select Multiple Sequence Alignment as the analysis type instead of Single Sequence.
Upload the file P07949.90-similar.msa.kinase.fasta and submit the job.
After submission, you will be redirected to the Token results page.

Solution

Once the prediction job has completed, the results will appear in the MSA Predictions table. It takes approximately less than two minutes to finish.
Click the View Results button to open the results page for the aligned kinase domain.

3.4.2 Understanding the aligned results as biological profiles

3.4.2.1 Exploring intrinsic flexibility and folding propensity

Explore the overview values

Explore the aligned values for P07949

Explore the values mapped to the 3D structure

3.4.2.2 Visualization of biophysical profiles

Explore the 90%-identity aligned backbone dynamics

Explore the 90%-identity aligned early folding events

Explore the 90%-identity aligned disorder propensities

Explore the 90%-identity aligned beta-aggregation propensities

In conclusion, as you see, working with 90%-identity sequences shows how well conserved are the biophysical profiles for this domain.

Interactive results

For further details: Open the results on the B2BTools platform.

3.5 Analysing the 50% identity aligned kinase domains

Given the 90% of identity does not allow us to see variability in the biophysical profiles, this section introduces the results for the sequences with 50% of identity extracted from UniProt.

Using the sequences as input for Clustal Omega to align the sequences, the next step is to extract the aligned domain via the Google Colab Notebook.

View the aligned kinase sequences

This is the alignment in FASTA format:

>tr|A0A0D9REZ9|A0A0D9REZ9_CHLSB Proto-oncogene tyrosine-protein kinase receptor Ret OS=Chlorocebus sabaeus OX=60711 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQDAPGPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A1U7Q3L7|A0A1U7Q3L7_MESAU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Mesocricetus auratus OX=10036 GN=Ret PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRDSRKIGPGYV---GGGGSRNSSSL
DHPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKKSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYHLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A2K5MKU6|A0A2K5MKU6_CERAT Proto-oncogene tyrosine-protein kinase receptor Ret OS=Cercocebus atys OX=9531 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A2K5V8L5|A0A2K5V8L5_MACFA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Macaca fascicularis OX=9541 GN=RET PE=3 SV=2
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A2K6CCS4|A0A2K6CCS4_MACNE Proto-oncogene tyrosine-protein kinase receptor Ret OS=Macaca nemestrina OX=9545 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A2R9B0Q5|A0A2R9B0Q5_PANPA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Pan paniscus OX=9597 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A2Y9LJM0|A0A2Y9LJM0_DELLE Proto-oncogene tyrosine-protein kinase receptor Ret OS=Delphinapterus leucas OX=9749 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A452UNU5|A0A452UNU5_URSMA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Ursus maritimus OX=29073 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A4W2I9B2|A0A4W2I9B2_BOBOX Proto-oncogene tyrosine-protein kinase receptor Ret OS=Bos indicus x Bos taurus OX=30522 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A6I9MCH9|A0A6I9MCH9_PERMB Proto-oncogene tyrosine-protein kinase receptor Ret OS=Peromyscus maniculatus bairdii OX=230844 GN=Ret PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRDSRKIGPGYV---GSGGSRNSSSL
DHPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKKSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A8B8VKU7|A0A8B8VKU7_BALMU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Balaenoptera musculus OX=9771 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A8C0AKK2|A0A8C0AKK2_BOSMU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Bos mutus grunniens OX=30521 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGILLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A8C0LH73|A0A8C0LH73_CANLU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Canis lupus dingo OX=286419 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A8C0TSX7|A0A8C0TSX7_CANLF Proto-oncogene tyrosine-protein kinase receptor Ret OS=Canis lupus familiaris OX=9615 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A8C2MD48|A0A8C2MD48_CRIGR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Cricetulus griseus OX=10029 GN=Ret PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRDSRKIGPGYV---GGGGSRNSSSL
DHPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKKSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A8C4LI87|A0A8C4LI87_EQUAS Proto-oncogene tyrosine-protein kinase receptor Ret OS=Equus asinus OX=9793 GN=RET PE=3 SV=2
LVLGKTLGEGEFGKVVEATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A8C6C2Y9|A0A8C6C2Y9_MONMO Proto-oncogene tyrosine-protein kinase receptor Ret OS=Monodon monoceros OX=40151 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A8C7BT94|A0A8C7BT94_NEOVI Proto-oncogene tyrosine-protein kinase receptor Ret OS=Neovison vison OX=452646 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGDV---GSGGSRSSSYL
DSPGERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A8C8XS60|A0A8C8XS60_PANLE Proto-oncogene tyrosine-protein kinase receptor Ret OS=Panthera leo OX=9689 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A8C9C6W7|A0A8C9C6W7_PHOSS Proto-oncogene tyrosine-protein kinase receptor Ret OS=Phocoena sinus OX=42100 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A8I3PW06|A0A8I3PW06_CANLF Proto-oncogene tyrosine-protein kinase receptor Ret OS=Canis lupus familiaris OX=9615 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A9V1FDH5|A0A9V1FDH5_PANPR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Panthera pardus OX=9691 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|F1MS00|F1MS00_BOVIN Proto-oncogene tyrosine-protein kinase receptor Ret OS=Bos taurus OX=9913 GN=RET PE=3 SV=3
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|F1RG22|F1RG22_PIG Proto-oncogene tyrosine-protein kinase receptor Ret OS=Sus scrofa OX=9823 GN=RET PE=3 SV=4
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRHSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|F6S299|F6S299_MACMU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Macaca mulatta OX=9544 GN=RET PE=3 SV=3
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|F6WV32|F6WV32_HORSE Proto-oncogene tyrosine-protein kinase receptor Ret OS=Equus caballus OX=9796 GN=RET PE=3 SV=2
LVLGKTLGEGEFGKVVEATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|G1MES8|G1MES8_AILME Proto-oncogene tyrosine-protein kinase receptor Ret OS=Ailuropoda melanoleuca OX=9646 GN=RET PE=3 SV=2
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|G1S142|G1S142_NOMLE Proto-oncogene tyrosine-protein kinase receptor Ret OS=Nomascus leucogenys OX=61853 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DDPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|G3QLZ3|G3QLZ3_GORGO Proto-oncogene tyrosine-protein kinase receptor Ret OS=Gorilla gorilla gorilla OX=9595 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|G3TIZ2|G3TIZ2_LOXAF Proto-oncogene tyrosine-protein kinase receptor Ret OS=Loxodonta africana OX=9785 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLHGACSQD--GPLFLIVEYAKYGSLRGFLRESRKVGPSYV---GSGGSRNSSYL
DNPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|H2NA70|H2NA70_PONAB Proto-oncogene tyrosine-protein kinase receptor Ret OS=Pongo abelii OX=9601 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|H2Q1T9|H2Q1T9_PANTR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Pan troglodytes OX=9598 GN=RET PE=3 SV=2
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|M3Z0Q6|M3Z0Q6_MUSPF Proto-oncogene tyrosine-protein kinase receptor Ret OS=Mustela putorius furo OX=9669 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLHGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGDV---GSGGSRSSSYL
DSPGERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>sp|P07949|RET_HUMAN Proto-oncogene tyrosine-protein kinase receptor Ret OS=Homo sapiens OX=9606 GN=RET PE=1 SV=3
LVLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYL
>tr|A0A2I3LXM4|A0A2I3LXM4_PAPAN Proto-oncogene tyrosine-protein kinase receptor Ret OS=Papio anubis OX=9555 GN=RET PE=3 SV=2
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A2I3TR47|A0A2I3TR47_PANTR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Pan troglodytes OX=9598 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A2Y9HVS1|A0A2Y9HVS1_NEOSC Proto-oncogene tyrosine-protein kinase receptor Ret OS=Neomonachus schauinslandi OX=29088 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A452SFQ5|A0A452SFQ5_URSAM Proto-oncogene tyrosine-protein kinase receptor Ret OS=Ursus americanus OX=9643 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A455AMB7|A0A455AMB7_PHYMC Proto-oncogene tyrosine-protein kinase receptor Ret OS=Physeter macrocephalus OX=9755 GN=RET PE=3 SV=2
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A4W2IB41|A0A4W2IB41_BOBOX Proto-oncogene tyrosine-protein kinase receptor Ret OS=Bos indicus x Bos taurus OX=30522 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A667GWR4|A0A667GWR4_LYNCA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Lynx canadensis OX=61383 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A667HC96|A0A667HC96_LYNCA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Lynx canadensis OX=61383 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A6J1ZQD7|A0A6J1ZQD7_ACIJB Proto-oncogene tyrosine-protein kinase receptor Ret OS=Acinonyx jubatus OX=32536 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A6J3Q7G4|A0A6J3Q7G4_TURTR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Tursiops truncatus OX=9739 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A6P5AYE8|A0A6P5AYE8_BOSIN Proto-oncogene tyrosine-protein kinase receptor Ret OS=Bos indicus OX=9915 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A7N9CS34|A0A7N9CS34_MACFA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Macaca fascicularis OX=9541 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A8B7TAT6|A0A8B7TAT6_HIPAR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Hipposideros armiger OX=186990 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVEATAFRLKGKAGYTTVAVKMLKENASPNELRDLLSEFNLLKQVN
HPNVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRDSRKAGPGYV---GSRGSHGSSYL
DNPDERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYL
>tr|A0A8B8U0M5|A0A8B8U0M5_CAMFR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Camelus ferus OX=419612 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASTSEMRDLQSEFNLLKQVN
HPQVIKLYGACSQG--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGDSRSSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A8B9YW94|A0A8B9YW94_BOSMU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Bos mutus grunniens OX=30521 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIES-LDHIYTTQSDVWSFGILLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A8C9JHD3|A0A8C9JHD3_PANTA Ret proto-onco OS=Panthera tigris altaica OX=74533 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GKAGQG------------------TGSCPVQAGGSGTSKAAACL
GHPRRRLPG--TEIRA-----------------------------ARRPGH-----WPER
AVSLCPLTGGGRGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A8D2EQF8|A0A8D2EQF8_THEGE Proto-oncogene tyrosine-protein kinase receptor Ret OS=Theropithecus gelada OX=9565 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A8U0NS18|A0A8U0NS18_MUSPF Proto-oncogene tyrosine-protein kinase receptor Ret OS=Mustela putorius furo OX=9669 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLHGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGDV---GSGGSRSSSYL
DSPGERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A9W2WDK0|A0A9W2WDK0_PHYMC Proto-oncogene tyrosine-protein kinase receptor Ret OS=Physeter macrocephalus OX=9755 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0AAV0A2E7|A0AAV0A2E7_PHORO Proto-oncogene tyrosine-protein kinase receptor Ret OS=Phodopus roborovskii OX=109678 GN=Ret PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRDSRKIGPGYV---GSGGSRNSSSL
DHPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKKSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|L8HXX9|L8HXX9_9CETA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Bos mutus OX=72004 GN=M91_06547 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGILLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A2I3H8C4|A0A2I3H8C4_NOMLE Proto-oncogene tyrosine-protein kinase receptor Ret OS=Nomascus leucogenys OX=61853 GN=RET PE=3 SV=1
E-------------------------KDRLQPALCCISENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DDPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A2J8IX93|A0A2J8IX93_PANTR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Pan troglodytes OX=9598 GN=CK820_G0052381 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A2R9AWY2|A0A2R9AWY2_PANPA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Pan paniscus OX=9597 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0A3Q7R7R3|A0A3Q7R7R3_CALUR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Callorhinus ursinus OX=34884 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A4U1ELZ6|A0A4U1ELZ6_MONMO Proto-oncogene tyrosine-protein kinase receptor Ret OS=Monodon monoceros OX=40151 GN=EI555_010818 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A4W2C1A7|A0A4W2C1A7_BOBOX Proto-oncogene tyrosine-protein kinase receptor Ret OS=Bos indicus x Bos taurus OX=30522 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A4X1U427|A0A4X1U427_PIG Proto-oncogene tyrosine-protein kinase receptor Ret OS=Sus scrofa OX=9823 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRHSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A4X1U649|A0A4X1U649_PIG Proto-oncogene tyrosine-protein kinase receptor Ret OS=Sus scrofa OX=9823 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRHSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A673TBL3|A0A673TBL3_SURSU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Suricata suricatta OX=37032 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A673TBM1|A0A673TBM1_SURSU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Suricata suricatta OX=37032 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A6B0RFM7|A0A6B0RFM7_9CETA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Bos mutus OX=72004 GN=E5288_WYG019359 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A6J2CLG4|A0A6J2CLG4_ZALCA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Zalophus californianus OX=9704 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A6P6C349|A0A6P6C349_PTEVA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Pteropus vampyrus OX=132908 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVEATAFRLKGKTGYMTVAVKMLKENASPNELRDLLSEFNLLKQVN
HPNVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRGSRNSSYL
DNPDERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMEKPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLEKMMVKRRDYL
>tr|A0A6P6C3D5|A0A6P6C3D5_PTEVA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Pteropus vampyrus OX=132908 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVEATAFRLKGKTGYMTVAVKMLKENASPNELRDLLSEFNLLKQVN
HPNVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRGSRNSSYL
DNPDERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMEKPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLEKMMVKRRDYL
>tr|A0A6P6HD24|A0A6P6HD24_PUMCO Proto-oncogene tyrosine-protein kinase receptor Ret OS=Puma concolor OX=9696 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A7J8GFA5|A0A7J8GFA5_ROUAE Proto-oncogene tyrosine-protein kinase receptor Ret OS=Rousettus aegyptiacus OX=9407 GN=HJG63_016583 PE=3 SV=1
LVLGKTLGEGEFGKVVEATAFRLKGKAGYTTVAVKMLKENASPNELRDLLSEFNLLKQVN
HPNVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRGSRNSNYL
DNPDERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMEKPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLEKMMVKRRDYL
>tr|A0A7N5KJC5|A0A7N5KJC5_AILME Proto-oncogene tyrosine-protein kinase receptor Ret OS=Ailuropoda melanoleuca OX=9646 GN=RET PE=3 SV=2
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A811YYL9|A0A811YYL9_NYCPR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Nyctereutes procyonoides OX=34880 GN=NYPRO_LOCUS12321 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFQLKGKAGYTTVAVKMLKENASPSELRDLLSELNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEHAKYGSLRGFLRESRKAGPGSV---GSGGSRSSSYL
DNPEERALTTGNLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSEGRIPVKWMAVESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKRGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADIGKDLEKMMVKNREYL
>tr|A0A811ZC71|A0A811ZC71_NYCPR Proto-oncogene tyrosine-protein kinase receptor Ret OS=Nyctereutes procyonoides OX=34880 GN=NYPRO_LOCUS19075 PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGSV---GSGGSRSSSYL
DNPEERALTTGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSEGRIPVKWMAVESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKRGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADIGKDLEKMMVKNREYL
>tr|A0A8C0RNG4|A0A8C0RNG4_CANLF Proto-oncogene tyrosine-protein kinase receptor Ret OS=Canis lupus familiaris OX=9615 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A8C0TXR9|A0A8C0TXR9_CANLF Proto-oncogene tyrosine-protein kinase receptor Ret OS=Canis lupus familiaris OX=9615 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A8D1M7Z5|A0A8D1M7Z5_PIG Proto-oncogene tyrosine-protein kinase receptor Ret OS=Sus scrofa OX=9823 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRHSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A8D1SMX3|A0A8D1SMX3_PIG Proto-oncogene tyrosine-protein kinase receptor Ret OS=Sus scrofa OX=9823 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRHSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0A8I3PV09|A0A8I3PV09_CANLF Proto-oncogene tyrosine-protein kinase receptor Ret OS=Canis lupus familiaris OX=9615 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A9B0H3A6|A0A9B0H3A6_ODORO Proto-oncogene tyrosine-protein kinase receptor Ret OS=Odobenus rosmarus divergens OX=9708 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0A9L0TGC7|A0A9L0TGC7_HORSE Proto-oncogene tyrosine-protein kinase receptor Ret OS=Equus caballus OX=9796 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVEATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0AAD4Y0S5|A0AAD4Y0S5_OVIAM Proto-oncogene tyrosine-protein kinase receptor Ret OS=Ovis ammon polii OX=230172 GN=MG293_020669 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0AAW0JHD4|A0AAW0JHD4_MYOGA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Myodes glareolus OX=447135 GN=U0070_021328 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRDSRKIGPGYV---GSGGSRNSSSL
DHPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKKSKGRIPVKWMAIESLFDHVYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0AB34GHJ1|A0AB34GHJ1_ESCRO Proto-oncogene tyrosine-protein kinase receptor Ret OS=Eschrichtius robustus OX=9764 GN=J1605_014116 PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|D2H6E6|D2H6E6_AILME Proto-oncogene tyrosine-protein kinase receptor Ret [Fragment] OS=Ailuropoda melanoleuca OX=9646 GN=PANDA_005545 PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|F6S7W7|F6S7W7_MACMU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Macaca mulatta OX=9544 GN=RET PE=3 SV=3
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|H9ZBZ9|H9ZBZ9_MACMU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Macaca mulatta OX=9544 GN=RET PE=2 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|H9ZC00|H9ZC00_MACMU Proto-oncogene tyrosine-protein kinase receptor Ret OS=Macaca mulatta OX=9544 GN=RET PE=2 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKVGPGYL---GSGGSRNSSSL
DHPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|W5P1A8|W5P1A8_SHEEP Proto-oncogene tyrosine-protein kinase receptor Ret OS=Ovis aries OX=9940 GN=RET PE=3 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---G----RNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0ABI7WFU7|A0ABI7WFU7_FELCA Uncharacterized protein OS=Felis catus OX=9685 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0ABK0KWL2|A0ABK0KWL2_RAT Ret proto-oncogene OS=Rattus norvegicus OX=10116 GN=Ret PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRDSRKIGPAYV---SSGGSRNSSSL
DHPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKKSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0ABM0HRK2|A0ABM0HRK2_CERSS Proto-oncogene tyrosine-protein kinase receptor Ret isoform X2 OS=Ceratotherium simum simum OX=73337 GN=LOC101401849 PE=4 SV=1
LVLGKTLGEGEFGKVVEATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0ABM0LFP2|A0ABM0LFP2_MICOH Proto-oncogene tyrosine-protein kinase receptor Ret OS=Microtus ochrogaster OX=79684 GN=Ret PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRDSRKIGPGYV---GSGGSRNSSSL
DHPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRD
VYEEDSYVKKSKGRIPVKWMAIESLFDHVYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0ABM2ENY1|A0ABM2ENY1_EQUPR Proto-oncogene tyrosine-protein kinase receptor Ret isoform X1 OS=Equus przewalskii OX=9798 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVEATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYL
>tr|A0ABM3NUI2|A0ABM3NUI2_ACIJB Proto-oncogene tyrosine-protein kinase receptor Ret isoform X2 OS=Acinonyx jubatus OX=32536 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0ABM3SCF4|A0ABM3SCF4_BALAC Proto-oncogene tyrosine-protein kinase receptor Ret OS=Balaenoptera acutorostrata OX=9767 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASASELRDLLSEFNLLTQVS
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSRDSRNSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKEEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0ABM4YZ43|A0ABM4YZ43_VULVU Proto-oncogene tyrosine-protein kinase receptor Ret isoform X1 OS=Vulpes vulpes OX=9627 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPHVIKLYGACSQD--GPLFLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRSSSYL
DNPEERALTMGDLISFAWQISRGMRYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYGLMLQCWKQEPDKRPVFADISKDLEKMMVKNRDYL
>tr|A0ABM5E203|A0ABM5E203_VICPA Proto-oncogene tyrosine-protein kinase receptor Ret OS=Vicugna pacos OX=30538 GN=RET PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASTSEMRDLQSEFNLLKQVN
HPQVIKLYGACSQG--GPLLLIVEYAKYGSLRGFLRESRKAGPGYM---GSGDNRSSSYL
DNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL
>tr|A0ABN8ZWN7|A0ABN8ZWN7_RANTA Uncharacterized protein OS=Rangifer tarandus platyrhynchus OX=3082113 GN=MRATA1EN1_LOCUS26358 PE=4 SV=1
LVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVN
HPQVIKLYGACSQD--GPLLLIVEYAKYGSLRGFLRESRKAGPGYV---GSGGSRNSSYL
DNLEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRD
VYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPE
RLFNLLKTGYRMERPDNCSEEMYSLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYL

Having the alignment ready, the B2BTools server can process this input file to return the biophysical profiles.

Explore the aligned backbone dynamics

Explore the aligned disorder

Explore the aligned early folding events

Interactive results

For further details: Open the results on the B2BTools platoform.

3.6 Connecting online resources

For a selected protein, a set of “external resources” will be shown in the left navigation bar if the header is recognised as a UniProt entry.

Hands-on: Finding phosphosites on Scop3P for P07949

Look for all the modified kinases on Scop3P at https://iomics.ugent.be/scop3p/search?q=kinase.
Find and select P07949 from the list.

Given the B2BTools page also includes the direct link, you can proceed with these steps:

1 Select the P07949 protein in the dropdown selector. 1. Clicking on the “Find PTMs on Scop3P” redirects you to the entry page on that resource: https://iomics.ugent.be/scop3p/index?protein=P07949.

Solution

🥪 LUNCH BREAK

Time to grab a coffee or tea. We’ll be back in one and a half hour…

To be continued: : Go to chapter 4

Next chapter explains how to explore the modifications (PTMs).

Jose Gavalda-Garcia, Adrián Díaz, and Wim Vranken. Bio2byte tools deployment as a python package and galaxy tool to predict protein biophysical properties. Bioinformatics (Oxford, England), 40(9):btae543, September 2024. URL: https://europepmc.org/articles/PMC11873786, doi:10.1093/bioinformatics/btae543. ↩
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Gabriele Orlando, Daniele Raimondi, Francesco Tabaro, Francesco Codicè, Yves Moreau, and Wim F Vranken. Computational identification of prion-like rna-binding proteins that form liquid phase-separated condensates. Bioinformatics, 35(22):4617–4623, 04 2019. URL: https://doi.org/10.1093/bioinformatics/btz274, arXiv:https://academic.oup.com/bioinformatics/article-pdf/35/22/4617/48978636/bioinformatics_35_22_4617.pdf, doi:10.1093/bioinformatics/btz274. ↩
Gabriele Orlando, Daniele Raimondi, Luciano Porto Kagami, and Wim F Vranken. Shiftcrypt: a web server to understand and biophysically align proteins through their nmr chemical shift values. Nucleic Acids Research, 48(W1):W36–W40, 05 2020. URL: https://doi.org/10.1093/nar/gkaa391, arXiv:https://academic.oup.com/nar/article-pdf/48/W1/W36/33432832/gkaa391.pdf, doi:10.1093/nar/gkaa391. ↩
Luciano Porto Kagami, Gabriele Orlando, Daniele Raimondi, Francois Ancien, Bhawna Dixit, Jose Gavaldá-García, Pathmanaban Ramasamy, Joel Roca-Martínez, Konstantina Tzavella, and Wim Vranken. B2btools: online predictions for protein biophysical features and their conservation. Nucleic Acids Research, 49(W1):W52–W59, 05 2021. URL: https://doi.org/10.1093/nar/gkab425, arXiv:https://academic.oup.com/nar/article-pdf/49/W1/W52/38842326/gkab425.pdf, doi:10.1093/nar/gkab425. ↩